Labanimal

February 2023 The papers behind the pod: Hobbiesiefken U et al. (2023). Rating enrichment items by female group-housed laboratory mice in multiple binary choice tests using an RFID-based tracking system. PloS one 18(1): e0278709. doi: 10.1371/journal.pone.0278709Petkov C et al. (2022). Unified ethical principles and an animal research ‘Helsinki’ declaration as foundations for international collaboration. Current Research in Neurobiology 3:100060. doi: 10.1016/j.crneur.2022.100060Weber E et al. (2023). Aggression in Group-Housed Male Mice: A Systematic Review. Animals 13(1):143. doi: 10.3390/ani13010143It’s the third Thursday of February and you’re listening to the final episode of 3 minute 3Rs, recapping the latest efforts to replace, reduce and refine the use of animals in research. Follow this link for the full transcript: https://www.nc3rs.org.uk/3-minute-3rs-podcast-february-2023-transcript Hosted on Acast. See

January 2023 The papers behind the pod: Cassotta M et al. (2022). A worldwide survey on the use of animal-derived materials and reagents in scientific experimentation. Engineering in Life Sciences 22(9):561-604. doi: 10.1002/elsc.202100167  Nuber M et al. (2022). Development of a 3D simulator for training the mouse in utero electroporation. PLOS One 17(12): e0279004. doi: 10.1371/journal.pone.0279004Dickmann J et al. (2022). Clicker Training Mice for Improved Compliance in the Catwalk Test. Animals 12(24): 3545. doi: 10.3390/ani12243545Happy New Year to all 3 Minute 3Rs listeners. It’s 2023, and we’re back with three more papers highlighting efforts to replace, reduce and refine the use of animals in research. Follow this link for the full transcript: https://www.nc3rs.org.uk/3-minute-3rs-podcast-january-2023-transcript  Hosted on Acast. See acast.com/privacy for more information.

December 2022The papers behind the pod: Menéndez ABC et al. (2022). Creating a kidney organoid-vasculature interaction model using a novel organ-on-chip system. Scientific Reports 12:20699. doi: 10.1038/s41598-022-24945-5Karp N et al. (2022). A qualitative study of the barriers to using blinding in in vivo experiments and suggestions for improvement. PLOS Biology 20(11): e3001873. doi: 10.1371/journal.pbio.3001873Talbot S et al. (2022). RELSA—A multidimensional procedure for the comparative assessment of well-being and the quantitative determination of severity in experimental procedures. Frontiers in Veterinary Science 9:937711. doi: 10.3389/fvets.2022.937711 It’s the third Thursday of December, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce and refine the use of animals in research. To round off 2022 we are highlighting a paper for each R.Follow this link for the f

November 2022The papers behind the pod: Aulehner K et al. (2022). Grimace scale, burrowing, and nest building for the assessment of post-surgical pain in mice and rats—A systematic review. Frontiers in Veterinary Science 9:930005. doi: 10.3389/fvets.2022.930005Hair K et al. (2022). ‘Living’ evidence frameworks for in vivo animal research: towards translational evidence-based medicine. BMJ Evidence-Based Medicine 2022;27:A17. doi: 10.1136/ebm-2022-EBMLive.31 Wong J et al. (2022). Electrochemically induced in vitro focal hypoxia in human neurons. Frontiers in Cell and Developmental Biology 10:968341. doi: 10.3389/fcell.2022.968341It’s the third Thursday of November, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce and refine the use of animals in research. Follow this link for the full transcript: https://www.nc3rs.org.uk/3-minute-3rs-podcast-november-2022-transcript Hosted o

October 2022The papers behind the pod: Piper, SK et al. (2022). Statistical review of animal trials—A guideline. Biometrical Journal https://doi.org/10.1002/bimj.202200061 Gargaté MJ et al. (2022). Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Model: Towards Less Dependence on the Mice Model. Pathogens https://doi.org/10.3390/pathogens11091038 Mieske P et al. et al. (2022). Bored at home?—A systematic review on the effect of environmental enrichment on the welfare of laboratory rats and mice. Frontiers in Veterinary Science https://doi.org/10.3389/fvets.2022.899219 It’s the third Thursday of October, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce and refine the use of animals in research. This month we have a paper on each R. Follow this link for the full transcript: https://nc3rs.org.uk/3-minute-3rs-podcast-october-2022-transcript Hosted on Acast. See acast.com/privacy for more information.

September 2022The papers behind the pod: Pinho RH et al. (2022). Validation of the rabbit pain behaviour scale (RPBS) to assess acute postoperative pain in rabbits (Oryctolagus cuniculus). PLoS ONE 17(5): e0268973. https://doi.org/10.1371/journal.pone.0268973 Stull C, Heagerty A and Coleman K (2022). Video Conference Technology as a Tool for Pair Introduction in Rhesus Macaques. Animals 12(14): e1783. https://doi.org/10.3390/ani12141783  Zentrich E et al. (2022). Postoperative Severity Assessment in Sheep. European Surgical Research, in press. https://doi.org/10.1159/000526058 It’s the third Thursday of September, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce, and refine the use of animals in research. This month we’re focusing on refinements for working with three different non-rodent species. Follow this link for the full transcript: https://nc3rs.org.uk/3-minute-3rs-podcast-september-2022-transcript Ho

August 2022The papers behind the pod: Arjmand S et al. (2022). Tips and traps for behavioural animal experimentation. Acta Neuropsychiatrica, in press. https://doi.org/10.1017/neu.2022.4Wrede P et al. (2022). Real-time 3D optoacoustic tracking of cell-sized magnetic microrobots circulating in the mouse brain vasculature. Science Advances 8(19). https://doi.org/10.1126/sciadv.abm9132 Russell A et al. (2022). Reduced housing density improves statistical power of murine gut microbiota studies. Cell Reports 39(6): e110783. https://doi.org/10.1016/j.celrep.2022.110783It’s the third Thursday of August, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce, and refine the use of animals in research. Follow this link for the full transcript: https://nc3rs.org.uk/3-minute-3rs-podcast-august-2022-transcript Our GDPR privacy policy was updated on August 8, 2022. Visit acast.com/privacy for more information.

July 2022The papers behind the pod: Ferreira DA et al. (2021). Alternative to Soft Lithography for the Fabrication of Organ-on-a-Chip Elastomeric-Based Devices and Microactuators. Advanced Science 8:2003273.  https://doi.org/10.1002/advs.202003273Eigel D et al. (2019). Cryogel scaffolds for regionally constrained delivery of lysophosphatidylcholine to central nervous system slice cultures: A model of focal demyelination for multiple sclerosis research. Acta Biomaterialia 97:216.  https://doi.org/10.1016/j.actbio.2019.08.030It’s the third Thursday of July, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce, and refine the use of animals in research. This month, we’ve got a special double feature highlighting the publications commended in the International 3Rs Prize, awarded by the NC3Rs and sponsored by GSK. Follow this link for the full transcript: https://nc3rs.org.uk/3-minute-3rs-podcast-july-2022-transcript See acast.com/privacy fo

June 2022The papers behind the pod: Williams WO and Baneux P (2022). Humane Intervention Points: Refining endpoint terminology to incorporate non-euthanasia intervention options to improve animal welfare and preserve experimental outcomes. Laboratory Animals, in press. https://doi.org/10.1177/00236772221090801 Osorio D et al. (2022). scTenifoldKnk: An efficient virtual knockout tool for gene function predictions via single-cell gene regulatory network perturbation. Patterns 3(3): e100434. https://doi.org/10.1016/j.patter.2022.100434Moudio S et al. (2022). Exposure of α-Synuclein Aggregates to Organotypic Slice Cultures Recapitulates Key Molecular Features of Parkinson's Disease. Frontiers in Neurology 13: e826102. https://doi.org/10.3389/fneur.2022.826102 It’s the third Thursday of June, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce and refine the use of animals in research. Follow this link for the

May 2022 The papers behind the pod:  Wang, Q et al. (2022). Assessment of a 3D neural spheroid model to detect pharmaceutical-induced neurotoxicity. ALTEX 39. https://doi.org/10.14573/altex.2112221 Reynolds P.S. (2022) Between two stools: preclinical research, reproducibility, and statistical design of experiments. BMC Res. Notes 15, 73.  https://doi.org/10.1186/s13104-022-05965-w Tappe-Theodor, A. et al. (2022) The “WWHow” Concept for Prospective Categorization of Post-operative Severity Assessment in Mice and Rats. Front. Vet. Sci. 9, 841431. https://doi.org/10.3389/fvets.2022.841431It’s the third Thursday of May, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce and refine the use of animals in research. This month, we’re bringing you a paper for each R. Follow this link for the full transcript: https://nc3rs.org.uk/3-minute-3rs-podcast-may-2022-transcript See acast.com/privacy for privacy and opt-out information.

April 2022The papers behind the pod:  Nigri M et al. (2022). Role of Environment and Experimenter in Reproducibility of Behavioral Studies With Laboratory Mice. Frontiers in Behavioural Neuroscience 16: e835444. https://doi.org/10.3389/fnbeh.2022.835444 Vámos A et al. (2022) Mitophagy Mediates the Beige to White Transition of Human Primary Subcutaneous Adipocytes Ex Vivo. Pharmaceuticals 15(3): 363. https://doi.org/10.3390/ph15030363 Ratuski AS and Weary DM (2022) Environmental Enrichment for Rats and Mice Housed in Laboratories: A Metareview. Animals 12(4): 414. https://doi.org/10.3390/ani12040414 It’s the third Thursday of April, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce and refine the use of animals in research. This month, we’re highlighting three papers focusing on replacement. Follow this link for the full transcript: https://nc3rs.org.uk/3-minute-3rs-podcast-april-

The papers behind the pod: 1.      Baran SW et al. (2022). Perspectives on the Evaluation and Adoption of Complex In Vitro Models in Drug Development: Workshop with the FDA and the Pharmaceutical Industry (IQ MPS Affiliate). ALTEX, in press. https://doi.org/10.14573/altex.21122032.      Borba JVB et al. (2022) STopTox: An in Silico Alternative to Animal Testing for Acute Systemic and Topical Toxicity. Environmental Health Perspectives 130(2). https://doi.org/10.1289/EHP93413.      van der Velden J et al. (2022) Animal models and animal-free innovations for cardiovascular research: current status and routes to be explored. Consensus document of the ESC working group on myocardial function and the ESC Working Group on Cellular Biology of the Heart. Cardiovascular Research, in press. https://doi.org/10.1093/cvr/cvab370 It’s the third Thursday of March, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts

February https://doi.org/10.1186/s12915-021-01184-0https://doi.org/10.1177/00236772211065920https://doi.org/10.1038/s41598-021-04502-2It’s the 3rd Thursday of February, and you’re listening to 3 Minute 3Rs. This month, we’re bringing you 3 refinement papers. Let’s start with rodent housing.Research rodents are conventionally housed in shoebox sized cages that limit their ability to perform natural behaviors such as nesting and burrowing. These restrictions are known to impair welfare, but could they even increase disease risk and shorten lifespans? A new meta-analysis compares the morbidity and mortality of rodents in conventional vs enriched housing. Conventional housing was found to significantly worsen disease severity for cancer, cardiovascular disease, stroke, anxiety, and depression. Conventional housing also increased all-cause mortality. In conclusion, conventional housing appears to be distressing enough to compromise rodent health in a biologically significant manner. This lends more evidence to the

The papers behind the pod:1. https://doi.org/10.7554/eLife.71601 & https://doi.org/10.7554/eLife.679952. https://doi.org/10.3389/fnins.2021.8056793. https://doi.org/10.1038/s41598-021-98356-3It’s the 3rd Thursday of January – happy new year! You’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce and refine the use of animals in research. Of course, we focus on those three Rs, but many have suggested adding a fourth R to the list: reproducibility. Designing experiments with reproducibility in mind is a key aspect of reducing unnecessary animal use, as well as being good for advancing science.In 2013 the Center of Open Science and Science Exchange began a collaboration to investigate the reproducibility of 193 experiments from 50 high-impact cancer biology papers. Over eight years of repeated experiments, they found that they could only reproduce 50 experiments from 23 papers, generally due to a lack of detail about the methods used or resources being unavailable. 15 of those 50

Dec 21 For our last episode of 2021, we’re highlighting two important topics in refinement: sedation and euthanasia. Sedation is necessary for zebrafish during procedures such as imaging, biopsy, and surgery to ensure animal welfare and high-quality science. But the effects of sedation can last beyond the administration period and should also be carefully considered. A paper by Gressler et al. explores the use of eugenol and propofol during a 3-hour sedation and their subsequent effects after a 1 hour washout period. Both drugs had effects on behavior and physiology even after the washout period. For behavior, in a novel tank test, eugenol was found to amplify diving response while propofol induced anti-anxiety responses. For physiology, both drugs caused alterations in gill structure. Clearly, as with other species, sedation of zebrafish can significantly affect behavior and physiology beyond the administration period. Therefore, sedation procedures must be carefully designed and reported to refine experimen

Nov 21The papers behind the pod:1.      A non-human primate in vitro functional assay for the early evaluation of TB vaccine candidates. npj Vaccines, January 2021. https://doi.org/10.1038/s41541-020-00263-72.      Inducible dominant negative ErbB2 rat spermatogonial line for generation of transgenic rat model and dissecting ERBB2 tyrosine kinase mediated pathways. Experimental Oncology, June 2019. https://doi.org/10.32471/exp-oncology.2312-8852.vol-41-no-2.130263.      Characterization of drug responses of mini patient-derived xenografts in mice for predicting cancer patient clinical therapeutic response. Cancer Communications, September 2018. https://doi.org/10.1186/s40880-018-0329-5Visit the AAALAC website to learn more about this year’s Global 3Rs Award winners: https://www.aaalac.org/awards/global-3rs-winners/It’s the third Thursday of November, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to

Oct 2021The papers behind the pod:1.      Professional Quality of Life in Research Involving Laboratory Animals. Animals 11(9), 2639 (2021) https://doi.org/10.3390/ani110926392.      Improving culture of care through maximising learning from observations and events: Addressing what is at fault. Laboratory Animals (2021). https://doi.org/10.1177%2F002367722110371773.      Epidemiology and reporting characteristics of preclinical systematic reviews. PLOS Biology (2021). https://doi.org/10.1371/journal.pbio.3001177 Transcript:It’s the 3rd Thursday of October, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce, and refine the use of animals in research. This month, we’re focusing on the people and strategies behind the science.First up, new research on compassion fatigue and its impact on those working with laboratory animals.Most professionals work in animal research because they want to

Sept 2021  You’re listening to the September episode of 3 Minute 3Rs. The papers behind the pod: Performance of preclinical models in predicting drug-induced liver injury in humans: a systematic review. Scientific Reports 11, 6403 (2021) https://doi.org/10.1038/s41598-021-85708-2 Ratlas-LH: An MRI template of the Lister hooded rat brain with stereotaxic coordinates for neurosurgical implantations Brain and Neuroscience Advances (2021) https://doi.org/10.1177/23982128211036332 O mouse, where art thou? The Mouse Position Surveillance System (MoPSS)—an RFID-based tracking system. Behavior Research Methods (2021) https://doi.org/10.3758/s13428-021-01593-7  [Transcript] It’s the 3rd Thursday of September, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce, and refine the use of animals in research. This month, we’ve got a paper for each R. Let’s get started with a r

You’re listening to the August episode of 3 Minute 3Rs!The papers behind the pod: Refinement of the spinal cord injury rat model and validation of its applicability as a model for memory loss and chronic pain. Heliyon 7, E07500 (2021) https://doi.org/10.1016/j.heliyon.2021.e07500 Strategies to encourage the adoption of social housing during cardiovascular telemetry recordings in non-rodents. Journal of Pharmalogical & Toxicological Methods 108, 106969 (2021). https://doi.org/10.1016/j.vascn.2021.106959 Refine acquisition of high-resolution chest CTs in macaques by free breathing. Laboratory Animals https://doi.org/10.1177%2F00236772211026562   Transcript:It’s the 3rd Thursday of August, and you are listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce, and refine the use of animals in research. This month, we’re bringing you three refinement papers. Let’s jump right in with a laminectomy. N

It’s the 3rd Thursday of July and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce, and refine the use of animals in research. The papers behind the pod: SARS-CoV-2 infects an upper airway model derived from induced pluripotent stem cells. Stem Cells https://doi.org/10.1002/stem.3422 A break from the pups: The effects of loft access on the welfare of lactating laboratory rats. PLos One https://doi.org/10.1371/journal.pone.0253020 3D printed rodent skin-skull-brain model: A novel animal-free approach for neurosurgical training. PLoS One https://doi.org/10.1371/journal.pone.0253477 This month, we’ll be covering welfare for rat moms and an animal-free option for practicing your surgical skills.  But first, let’s hear about an in vitro model for COVID-19 research.  [NC3Rs] As researchers around the world fight against COVID-19, in vitro models continue